R-loopBase is an open source platform, aiming to provide the most comprehensive annotations for R-loops and associated regulatory proteins.

The controversial genomic localization of R-loops and incomplete understanding of R-loop regulatory network raise great challenges for R-loop research. To tackle these pressing issues, we developed R-loopBase database by systematic integration of a huge amount of genomics and literature data. A reference set of human R-loop zones was computed with confidence scores (for more information regarding confidence scores, please refer to Q5). With literature mining and multi-omics analyses, we manually curated the most comprehensive list of R-loop regulating proteins in multiple species to date, and specifically characterize R-loops targeted by individual regulators in human. Finally, we integrate billions of functional genomic annotations, and develop interactive interfaces to search, visualize, evaluate, download and analyze R-loops and R-loop regulators in the well-annotated genomic context. R-loopBase allows users even with little bioinformatics background to leverage these data for their own research. It will be continueously updated by joint efforts from R-loopBase team and researchers who are willing to contribute to R-loopBase development.