A protein is considered as an R-loop regulator if it binds to, stabilizes, resolves or degrades RNA:DNA hybrids, or levels of R-loops or RNA:DNA hybrids are changed upon chemical or genetic manipulation of the protein. Such effects can be directly or indirectly exerted by the R-loop regulator.
By searching literatures from PubMed database, we manually curated lists of R-loop regulators for four species available on and before March 31st, 2021. A total of 1,185 proteins in human, 24 in mouse, 63 in yeast (Saccharomyces cerevisiae) and 21 in E. coli were collected as R-loop regulatory proteins. Examplified with validated human R-loop regulators, we performed PPI network analysis to provide a global overview of functional relationship of R-loop regulators. Seven distinct clusters were readily identified, and GO enrichment analysis revealed cluster-specific enrichment of biological processes.
If you find any R-loop regulator mis-annotated or completely missed by R-loopBase, please kindly let us know. We will update it after further inspection by R-loopBase team.