ID:AGPD1_HUMAN DESCRIPTION: RecName: Full=Hydroxylysine kinase; Short=5-hydroxy-L-lysine kinase; EC=2.7.1.81; AltName: Full=Aminoglycoside phosphotransferase domain-containing protein 1; FUNCTION: Catalyzes the GTP-dependent phosphorylation of 5- hydroxy-L-lysine. CATALYTIC ACTIVITY: GTP + 5-hydroxy-L-lysine = GDP + 5- phosphonooxy-L-lysine. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=280 uM for (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP); KM=7.6 uM for (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP); KM=810 uM for ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L- lysine); KM=3.5 uM for GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L- lysine); Vmax=4.95 umol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP); Vmax=0.49 umol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP); Vmax=5.28 umol/min/mg enzyme toward ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine); Vmax=0.57 umol/min/mg enzyme toward GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine); SUBCELLULAR LOCATION: Cytoplasm (Potential). SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
Protein Domain and Structure Information
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