Description

This track displays R-loop zones identified by integrating all mapped R-loop peaks from control cells across multiple technologies. Peaks were first merged and strand-assigned, and the resulting zones were stratified into confidence levels based on the minimum number of supporting technologies, with higher levels indicating greater conservation. This stratified view allows users to evaluate the reproducibility and robustness of R-loop sites, ranging from permissive to highly stringent calls.

Methods

First, stranded R-loop peaks were merged as R-loop zones on Watson or Crick strand separately. Non-stranded R-loops, if overlapped with stranded R-loop peaks, were also assigned to Watson or Crick R-loop zones accordingly. The remaining non-stranded R-loop peaks were merged as non-stranded R-loop zones. Second, the resulting R-loop zones were further partitioned into sub-regions of n different confidence levels, where n is the minimal number of technologies they were detected by. For example, R-loop zones of level 3 were those co-detected by >=3 technologies. The higher the level is, the more conservative the R-loop zones are.